Five new specimens and 49 echolocation records (< 0.2% of all echolocation sequences recorded) identified as eastern pipistrelle, Pipistrellus subflavus (F. Cuvier) are documented in New Brunswick. Previously this bat was known in New Brunswick from a single specimen discovered over-wintering in a natural limestone cave. New records show the eastern pipistrelle confined to the Fundy coast of New Brunswick, but over-wintering in both natural caves and an abandoned graphite mine. In New Brunswick, where they have been recorded hibernating with congregations of Myotis species, eastern pipistrelles comprised less than 1.5% of the bats present. Pipistrelle echolocation sequences that are the first reports for the species outside winter roosting habitat in New Brunswick indicate a strong preference for feeding over water. Both echolocation sequences and specimen records suggest that the eastern pipistrelle is of very rare but regular occurrence in southern coastal New Brunswick. The species' provincial distribution may be influenced by the availability of caves and mines suitable for over-wintering.